MDH2 Antibody

SKU: orb389375

Description

Goat polyclonal antibody to MDH2

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:8000, WB: 0.01-0.03 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human, Mouse, Porcine, Rat
• Predicted Reactivity: Bovine
• Application Notes:
  WB: Approx 35kDa band observed in lysates of cell lines HeLa, HepG2 and NIH3T3 (calculated MW of 35.5kDa according to Human NP_005909.2 and 35.6kDa according to Mouse NP_032643.2 ).

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: MDH2
• Protein Sequence: TRDDLFNTNAT
• Molecular Weight: 35.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MDH2, malate dehydrogenase 2, NAD (mitochondrial), M-MDH, MDH, MGC:3559, MOR1, malate dehydrogenase, mitochondrial

0.1 µg/ml staining of HeLa (A), HepG2 (B) and NIH3T3 (C) lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

0.01 µg/ml staining of Human (A), Mouse (B), Rat (C) and Pig (D) Heart lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

5 µg/ml staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

MDH2

NCBI Gene ID

4191