Goat anti-MDA5 / Ifih1 (mouse) Antibody

• Catalog Number: orb19561
• Category: Antibodies
• Description: Goat polyclonal antibody to Ifih1
• Target: MDA5 / Ifih1 (mouse)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Mouse
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: KQKLRNNDLKKQN
• RRID: AB_10751084
• MW: 116
• Tested applications: ELISA
• Dilution range: ELISA: 1:16000, WB: 1 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 80kDa band in Mouse Brain lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 116kDa according to NP_082111.1. The 80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti Ifih1 antibody, anti interferon induced with
• Note: For research use only