Goat anti-MAPK3/ERK1 Antibody

SKU: orb18996

Description

Goat polyclonal antibody to MAPK3

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution Range: ELISA: 1:4000, WB: 0.1-1 μg/ml, IHC-P: 10ug/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 45kDa band observed in Human Brain lysates, Kidney fibroblast HEK293 lysates and epidermoid fibroblast A431 lysates (calculated MW of 43.1kDa according to NP_002737.1). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: MAPK3/ERK1
• Protein Sequence: GGEPRRTEGVGP
• Molecular Weight: 43.1; 40.1; 38.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti MAPK3 antibody, anti ERK1 antibody, anti mitogen-activated protein kinase 3 antibody, anti HGNC:6877 antibody, anti P44ERK1 antibody, anti P44MAPK antibody, anti PRKM3 antibody, anti protein kinase, mitogen-activated 3 (MAP kinase 3 p44) antibody, anti HS44KDAP antibody, anti HUMKER1A antibody, anti MGC20180 antibody, anti OTTHUMP00000174538 antibody, anti extracellular signal-regulated kinase 1 antibody, anti extracellular signal-related kinase 1 antibody

orb18996 (0.3ug/ml) staining of 293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing ERK1 (RC204196) and probed with orb18996 (mock transfection in second lane), tested by Origene

Quick Database Links

Gene Symbol

MAPK3/ERK1

NCBI Gene ID

5595