Goat anti-MAP3K6 Antibody

• Catalog Number: orb20336
• Category: Antibodies
• Description: Goat polyclonal antibody to MAP3K6
• Target: MAP3K6
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: RAILAQRAGSTP
• RRID: AB_10753751
• MW: 143
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:8000, WB: 0.3-1 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 140kDa band observed in Rat Heart lysates (calculated MW of 143kDa according to Human NP_004663.3 and Rat NP_001101379.1). Recommended concentration: 0.3-1 μg/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti MAP3K6 antibody, anti mitogen-activated prote
• Note: For research use only
• Entrez: 9064

orb20336 (0.3ug/ml) staining of Rat Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.