MAP3K6 Antibody

SKU: orb20336

Description

Goat polyclonal antibody to MAP3K6

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:8000, WB: 0.3-1 μg/ml
• Reactivity: Rat
• Predicted Reactivity: Bovine, Canine, Human, Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 140kDa band observed in Rat Heart lysates (calculated MW of 143kDa according to Human NP_004663.3 and Rat NP_001101379.1). Recommended concentration: 0.3-1 μg/ml. An additional band of unknown identity was also consistently observed at 28kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: MAP3K6
• Molecular Weight: 143
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti MAP3K6 antibody, anti mitogen-activated protein kinase kinase kinase 6 antibody, anti ASK2 antibody, anti MAPKKK6 antibody, anti MGC125653 antibody, anti MGC20114 antibody, anti OTTHUMP00000005063 antibody, anti apoptosis signal-regulating kinase 2 antibody

orb20336 (0.3ug/ml) staining of Rat Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

MAP3K6

NCBI Gene ID

9064