Goat anti-MAD4 / MXD4 Antibody

• Catalog Number: orb18793
• Category: Antibodies
• Description: Goat polyclonal antibody to MXD4
• Target: MAD4 / MXD4
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: GPHCRRLGRPALS
• RRID: AB_10750739
• MW: 23.5
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:16000, WB: 0.5-2 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 22kDa band observed in human kidney lysates (predicted MW of 24kDa according to NP_006445). Recommended for use at 0.5-2 μg/ml. Please note the appearance of this band was blocked by incubation with the immunising peptide.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti MXD4 antibody, anti MAD4 antibody, anti MAX d
• Note: For research use only
• Entrez: 10608

orb18793 staining (0.5ug/ml) of human kidney lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.