LXR beta/NR1H2 Antibody

SKU: orb18918

Description

Goat polyclonal antibody to NR1H2

Research Area

Cancer

Images & Validation

• Tested Applications: ELISA, FC, IF
• Dilution Range: ELISA: 1:128000, IHC-P: 10ug/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Spleen shows nucleolar staining. Recommended concentration, 1-2ug/ml.WB: Experiments gave a band at approx 80kDa and a weak band at appr. 60-kDa in human bone marrow lysate after 1 μg/ml antibody staining. These bands correspond to earlier findings with different antibodies (PMID: 12393874-60kDa and other unpublished antibodies-80kDa). This protein has a calculated MW of 51.0kDa according to NP_009052. Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: LXR beta / NR1H2
• Protein Sequence: SSPTTSSLDTPLPGC
• Molecular Weight: 51
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti NR1H2 antibody, anti LXR-b antibody, anti NER antibody, anti UNR antibody, anti NER-I antibody, anti RIP15 antibody, anti nuclear receptor subfamily 1, group H, member 2 antibody, anti ubiquitously-expressed nuclear receptor antibody, anti LXRB antibody, anti LX receptor beta antibody, anti liver X receptor beta antibody, anti liver X receptor-beta antibody, anti nuclear orphan receptor LXR-beta antibody, anti oxysterols receptor LXR-beta antibody, anti steroid hormone-nuclear receptor NER antibody

Immunohistochemical staining of paraffin embedded Human Spleen using NR1H2 antibody

Immunohistochemical staining of Human Uterus using NR1H2 antibody

Western blot analysis of Human Bone Marrow lysate using NR1H2 antibody

Immunofluorescence analysis of U2OS cells using NR1H2 antibody

Flow cytometric analysis of HeLa cells using NR1H2 antibody

Quick Database Links

Gene Symbol

LXR beta / NR1H2

NCBI Gene ID

7376