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LSP1 (aa261-275) Antibody

SKU: orb20457

Description

Goat polyclonal to LSP1

Research Area

Epigenetics

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeELISA: 1:16000, WB: 1-3 μg/ml
ReactivityHuman
Predicted ReactivityBovine, Canine
Application Notes
This antibody is expected to recognize both reported isoforms (NP_002330.1; NP_001013271.1). Reported variants represent identical protein: NP_001013273.1, NP_001013271.1, NP_001013272.1ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 55kDa band observed in Human Lymph Node, Tonsil and in Human Peripheral Blood Mononucleocytes lysates (calculated MW of 37.2kDa according to NP_002330.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Liu et al, J Exp Med. 2005 Feb 7;201(3):409-18. PMID: 15684321). Recommended concentration: 1-3 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetLSP1 (aa261-275)
Protein SequenceASTKSRWETGEVQAQ
Molecular Weight37.2; 30.2
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti LSP1 antibody, anti lymphocyte-specific protein 1 antibody, anti WP34 antibody, anti pp52 antibody, anti 47 kDa actin binding protein antibody, anti 52 kDa phosphoprotein antibody, anti F-actin binding and cytoskeleton associated protein antibody, anti leufactin (leukocyte F-actin binding protein) antibody, anti leukocyte-specific protein 1 antibody, anti lymphocyte-specific antigen WP34 antibody, anti lymphocyte-specific protein pp52 antibody
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LSP1 (aa261-275) Antibody

orb20457 (1ug/ml) staining of Peripheral Blood Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

LSP1 (aa261-275) Antibody (orb20457)

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Available Sizes

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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