Goat anti-LRRK2 / PARK8 (near C Terminus) Antibody

• Catalog Number: orb18949
• Category: Antibodies
• Description: Goat polyclonal antibody to LRRK2
• Target: LRRK2 / PARK8 (near C Terminus)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: CELAEKMRRTSV
• RRID: AB_10750505
• MW: 286
• Tested applications: ELISA, IF, WB
• Dilution range: ELISA: 1:128000, IHC-P: 10 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Brain (hippocampus) shows staining of neuronal cytoplasm. Recommended concentration, 1-2ug/ml.WB: This product has been successfully used in both Western blot and immunohistochemistry on human brain: Alegre-Abarrategui J et al., Neuropathol Appl Neurobiol. 2007 Oct 26; PMID: 17971075. Recommended concentration: 0.3-1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti LRRK2 antibody, anti PARK8 antibody, anti ROC
• Research Area: Autophagic, Cancer, Epigenetics, Neuroscience, Sig
• Note: For research use only
• Entrez: 120892

Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing vesicle staining. The nuclear stain is DAPI (blue).

Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml). Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear and vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).