LRP6 Antibody

SKU: orb18907

Description

Goat polyclonal antibody to LRP6

Research Area

Developmental Biology, Epigenetics, Neuroscience, Signaling Pathways, Stem Cells, Wnt

Images & Validation

• Tested Applications: ELISA, FC, IHC
• Dilution Range: ELISA: 1:64000, WB: 0.1 μg/ml, IHC-P: 4-6 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Kidney shows luminar membrane staining in DCT. Recommended concentration, 4-6ug/ml.WB: Preliminary experiments gave bands at approx 50kDa and 35kDa in Human Adipose lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 180kDa according to NP_002327.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: LRP6
• Protein Sequence: CMTSVATAKGYTSDL
• Molecular Weight: 180
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti LRP6 antibody, anti low density lipoprotein receptor-related protein 6 antibody, anti low density lipoprotein-related protein 6 antibody

Immunohistochemical staining of Human Kidney using LRP6 antibody.

Flow cytometric analysis of paraformaldehyde fixed K562 cells using LRP6 antibody.

Quick Database Links

Gene Symbol

LRP6

NCBI Gene ID

4040