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Goat anti-LNK / SH2B3 Antibody

Catalog Number: orb18867

DispatchUsually dispatched within 5-10 working days
$ 500.00
Catalog Numberorb18867
CategoryAntibodies
DescriptionGoat polyclonal antibody to SH2B3
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, WB
ReactivityHuman
Dilution rangeELISA: 1:64000, WB: 0.3-1.0 μg/ml
ConjugationUnconjugated
MW63.2
TargetLNK / SH2B3
Entrez10019
Protein SequenceNGPALQPSSPC
RRIDAB_10753001
StorageAliquot and store at -20°C. Minimize freezing and thawing.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Alternative namesanti SH2B3 antibody, anti LNK antibody, anti SH2B
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NoteFor research use only
Application notesELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 70kDa band observed in lysates of cell line MOLT4 (calculated MW of 63.2kDa according to NP_005466.1). In transfected HEK293 transiently expressing SH2B3 a band of approx. 65kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1.0 μg/ml.
Expiration Date12 months from date of receipt.
Goat anti-LNK / SH2B3 Antibody

orb18867 (0.3ug/ml) staining of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Goat anti-LNK / SH2B3 Antibody

HEK293 overexpressing SH2B3 (RC218359) and probed with orb18867 (mock transfection in first lane), tested by Origene.