LMO7/FBXO20 Antibody

• Catalog Number: orb18591
• Category: Antibodies
• Description: Goat polyclonal antibody to LMO7
• Target: LMO7 / FBXO20
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: ESTCVSASLPRS
• RRID: AB_10749319
• MW: 89.7
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:32000, WB: 0.5-2 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 70-75kDa band and approx 30kDa band observed in Human Lung lysates (predicted MWs of 92kDa according to AAD33924.1 - however our result agrees with Putilina et al as below). A minor band of unknown identity was also consistently observed at approx 50kDa. Recommended for use at 0.5-2 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti LMO7 antibody, anti FBXO20 antibody, anti LOM
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 4008

orb18591 staining (1ug/ml) of Human Lung lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.