LIMP2/SCARB2 Antibody

SKU: orb20222

Description

Goat polyclonal antibody to SCARB2

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:4000, WB: 0.1-0.3 μg/ml, IHC-P: 3-5µg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  IHC: In paraffin embedded Human Cerebral Cortex shows lysosomal staining. Recommended concentration, 3-5μg/ml.WB: Approx. 80kDa band observed in Human Brain (Cortex and Cerebellum) lysates (calculated MW of 54.3kDa according to NP_005497.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Fujita et al, Biochem Biophys Res Commun. 1992 Apr 30;184(2):604-11.; PMID: 1374238). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: LIMP2 / SCARB2
• Molecular Weight: 54.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti SCARB2 antibody, anti scavenger receptor class B, member 2 antibody, anti AMRF antibody, anti HLGP85 antibody, anti LIMPII antibody, anti SR-BII antibody, anti 85 kDa lysosomal sialoglycoprotein scavenger receptor class B, member 2 antibody, anti lysosomal integral membrane protein II antibody

Western blot analysis of human cerebral cortex lysate (35µg protein in RIPA buffer) using LIMP2 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded human cerebral cortex tissue using LIMP2 antibody

Quick Database Links

Gene Symbol

LIMP2 / SCARB2

NCBI Gene ID

950