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Item 1 of 3
Goat anti-Leptin Receptor Antibody
Catalog Number: orb19243
| Catalog Number | orb19243 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to Leptin Receptor |
| Target | Leptin Receptor |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Canine, Human |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Protein Sequence | TQDDIEKHQSDAG |
| RRID | AB_10762720 |
| MW | 132; 102; 109 |
| Tested applications | ELISA, FC, IHC, WB |
| Dilution range | ELISA: 1:16000, WB: 0.1-1 μg/ml, IHC-P: 4 μg/ml |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 150kDa band observed in Human Brain lysates (calculated MW of 132kDa according to NP_002294.2). Recommended concentration: 1-3 μg/ml. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti CD 295 antibody, anti CD295 antibody, anti LE Read more... |
| Note | For research use only |
| Entrez | 3953 |
Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line) permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488® conjugated secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Immunofluorescence analysis of paraformaldehyde fixed U251. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor® 488 secondary antibody (1:2000 dilution), showing cytoplasmic and vesicles staining. The nuclear stain is DAPI (blue). Negative control: Anti-Goat IgG followed by Alexa Fluor® 488 secondary antibody.
Immunofluorescence analysis of paraformaldehyde fixed HEK293. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor® 488 secondary antibody (1:2000 dilution), showing cytoplasmic and vesicles staining. The nuclear stain is DAPI (blue). Negative control: Anti-Goat IgG followed by Alexa Fluor® 488 secondary antibody.
