Goat anti-KU70 / XRCC6 Antibody

• Catalog Number: orb19544
• Category: Antibodies
• Description: Goat polyclonal antibody to XRCC6
• Target: KU70 / XRCC6
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: YKTEGDEEAEEEQ
• RRID: AB_10753564
• MW: 69.8
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:2000, WB: 0.1-0.3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 70kDa band observed in lysates of cell lines A431, HeLa and HepG2 (calculated MW of 69.8kDa according to NP_001460.1). In transfected HEK293 transiently expressing KU70 a band of approx. 70kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.1-0.3 μg/ml.
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti XRCC6 antibody, anti KU70 antibody, anti X-ra
• Note: For research use only
• Entrez: 2547

orb19544 (0.1ug/ml) staining of HeLa cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing KU70 (RC204048) and probed with orb19544 (mock transfection in first lane), tested by Origene.