KMT2B/MLL4 Antibody

SKU: orb18718

Description

Goat polyclonal antibody to MLL4

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:128000, IHC-P: 5-10 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: Immunohistochemistry: In paraffin embedded Human Testis shows cytoplasm staining of spermatids. Recommended concentration, 5-10ug/ml.WB: Preliminary experiments gave no signal but low background in Human Heart lysates at up to 1 μg/ml. However this used our routine western blotting protocol which we would not expect to reliably detect proteins as large as the predicted size of approx. 312KDa according to NP_055542. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: KMT2B/MLL4
• Protein Sequence: CNCGAKRCRRFLN
• Molecular Weight: 294
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti MLL4 antibody, anti myeloid/lymphoid or mixed-lineage leukemia 4 antibody, anti HRX2 antibody, anti MLL2 antibody, anti KIAA0304 antibody, anti KIAA0304 gene product antibody

orb18718 (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

KMT2B/MLL4

NCBI Gene ID

9757