Goat anti-KMT2B/MLL4 Antibody

• Catalog Number: orb18718
• Category: Antibodies
• Description: Goat polyclonal antibody to MLL4
• Target: KMT2B/MLL4
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Canine, Human, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: CNCGAKRCRRFLN
• RRID: AB_10747007
• MW: 294
• Tested applications: ELISA, IHC
• Dilution range: ELISA: 1:128000, IHC-P: 5-10 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: Immunohistochemistry: In paraffin embedded Human Testis shows cytoplasm staining of spermatids. Recommended concentration, 5-10ug/ml.WB: Preliminary experiments gave no signal but low background in Human Heart lysates at up to 1 μg/ml. However this used our routine western blotting protocol which we would not expect to reliably detect proteins as large as the predicted size of approx. 312KDa according to NP_055542. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti MLL4 antibody, anti myeloid/lymphoid or mixed
• Note: For research use only
• Entrez: 9757

orb18718 (5ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.