Klotho Antibody

SKU: orb19130

Description

Goat polyclonal antibody to Klotho

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:4000, WB: 1 μg/ml, IHC-P: 4-6 μg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.IHC: In paraffin embedded Human Breast shows cytoplasm staining in luminla epithelial cells of ducts, while Human Placenta shows membranous staining. Recommended concentration, 4-6ug/ml.WB: Preliminary experiments in human brain, duodenum, placenta and prostate lysates gave no specific signal but low background (at antibody concentration up to 1 μg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Klotho
• Protein Sequence: KRDDAKYMYYLKK
• Molecular Weight: 116; 62.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti KL antibody, anti KLOT_HUMAN antibody, anti Klotho peptide antibody

orb19130 (4ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

orb19130 (4ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Quick Database Links

Gene Symbol

Klotho

NCBI Gene ID

9365