Goat anti-Klotho Antibody

• Catalog Number: orb19130
• Category: Antibodies
• Description: Goat polyclonal antibody to Klotho
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Human, Mouse, Rat
• Dilution range: ELISA: 1:4000, WB: 1 μg/ml, IHC-P: 4-6 μg/ml
• Conjugation: Unconjugated
• MW: 116; 62.1
• Target: Klotho
• Entrez: 9365
• Protein Sequence: KRDDAKYMYYLKK
• RRID: AB_10751669
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti KL antibody, anti KLOT_HUMAN antibody, anti K
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.IHC: In paraffin embedded Human Breast shows cytoplasm staining in luminla epithelial cells of ducts, while Human Placenta shows membranous staining. Recommended concentration, 4-6ug/ml.WB: Preliminary experiments in human brain, duodenum, placenta and prostate lysates gave no specific signal but low background (at antibody concentration up to 1 μg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates
• Expiration Date: 12 months from date of receipt.

orb19130 (4ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

orb19130 (4ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.