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| Catalog Number | orb20525 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal to Klk1 |
| Target | Klk1 (mouse) |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Predicted Reactivity | Mouse |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Protein Sequence | FNTWIRETMAEND |
| RRID | AB_10928968 |
| MW | 28.8 |
| Tested applications | ELISA |
| Dilution range | ELISA: 1:4000, WB: 0.3 μg/ml |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Preliminary experiments gave an approx 55kDa band in Mouse Liver, Heart and Testis lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 28.8kDa according to NP_034769.4. The 55kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | anti Klk1 antibody, anti kallikrein 1 antibody, an Read more... |
| Research Area | Cardiovascular Disease, Epigenetics, Neuroscience |
| Note | For research use only |
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