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keratin 18 Antibody

SKU: orb176723

Description

Goat polyclonal antibody to Cytokeratin 18

Research Area

Epigenetics, Infectious Diseases

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
ReactivityHuman, Porcine
Predicted ReactivityBovine, Canine, Mouse, Rat
Application Notes
Reported variants represent identical protein: NP_000215.1, NP_954657.1.ELISA: antibody detection limit dilution 1:32000.WB: Approx 48kDa band observed in lysates of cell lines A431, HeLa, MCF7 (calculated MW of 48.1kDa according to NP_000215.1) and approx 45kDa in lysates of Pig Liver (calculated MW of 47.4kDa according to XP_005652636.2). Recommended concentration: 0.1-0.3µg/ml.

Key Properties

ClonalityPolyclonal
Targetkeratin 18
Protein SequenceEELDKYWSQQIE
Molecular Weight48.1
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti-CYK 18 antibody, anti-CYK18 antibody, anti-CK 18 antibody, anti-CK18 antibody, anti-Cytokeratin 18 antibody, anti-Cytokeratin-18 antibody, anti-K 18 antibody, anti-K18 antibody, anti-KA18 antibody, anti-Keratin 18 antibody, anti-Keratin D antibody, anti-keratin, type I cytoskeletal 18 antibody, anti-Keratin-18 antibody, anti-Krt18 antibody, anti-Cell proliferation inducing gene 46 protein antibody

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

keratin 18 Antibody (orb176723)

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Available Sizes

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100 μg
$ 560.00
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