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Item 1 of 2
| Catalog Number | orb19764 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to Kcnj11 |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC, WB |
| Reactivity | Canine, Human, Mouse, Rat |
| Dilution range | ELISA: 1:64000, WB: 0.01-0.03 μg/ml, IHC-P: 3-5µg/ml |
| Conjugation | Unconjugated |
| MW | 43.6 |
| Target | Kcnj11 / Kir6.2 |
| Entrez | 3767 |
| Protein Sequence | ERRARFVSKKGNC |
| RRID | AB_10750452 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | anti KCNJ11 antibody, anti Kir6.2 antibody, anti p Read more... |
| Note | For research use only |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Pancreas shows variable staining across the islet of Langerhans. Recommended concentration, 3-5ug/ml.WB: Approx 45kDa band observed in Human Skeletal Muscle lysates (calculated MW of 43.6kDa according to NP_034732.1). Recommended concentration: 0.01-0.03 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
Shimbo, Tomonori et al. In vitro effect of nicorandil on the carbachol-induced contraction of the lower esophageal sphincter of the rat J Pharmacol Sci, 131, 267-274 (2016)
orb19764 (0.01ug/ml) staining of Human Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb19764 (3.8ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
