Kcnj11/Kir6.2 Antibody

SKU: orb19764

Description

Goat polyclonal antibody to Kcnj11

Research Area

Cardiovascular Disease, Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:64000, WB: 0.01-0.03 μg/ml, IHC-P: 3-5µg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Pancreas shows variable staining across the islet of Langerhans. Recommended concentration, 3-5ug/ml.WB: Approx 45kDa band observed in Human Skeletal Muscle lysates (calculated MW of 43.6kDa according to NP_034732.1). Recommended concentration: 0.01-0.03 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Kcnj11 / Kir6.2
• Protein Sequence: ERRARFVSKKGNC
• Molecular Weight: 43.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti KCNJ11 antibody, anti Kir6.2 antibody, anti potassium inwardly-rectifying channel, subfamily J, member 11 antibody, anti mBIR antibody, anti AI842722 antibody, anti AW491124 antibody, anti potassium inwardly rectifying channel, subfamily J, member 11 antibody, anti KATP antibody, anti KATP channel antibody

orb19764 (0.01ug/ml) staining of Human Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19764 (3.8ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

Kcnj11 / Kir6.2

NCBI Gene ID

3767

Shimbo, Tomonori et al. In vitro effect of nicorandil on the carbachol-induced contraction of the lower esophageal sphincter of the rat J Pharmacol Sci, 131, 267-274 (2016)

PubMed: 27562702