Goat anti-KCNJ11 / KATP / Antibody

SKU: orb19676

Description

Goat polyclonal antibody to KCNJ11

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 3-5µg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Pancreas shows variable staining across the islet of Langerhans. Recommended concentration, 3-5ug/ml.WB: Approx 45-48kDa band observed in Human Skeletal Muscle lysates (calculated MW of 43.5kDa according to NP_000516.3). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: KCNJ11 / KATP /
• Molecular Weight: 43.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti KCNJ11 antibody, anti IKATP antibody, anti potassium inwardly-rectifying channel, subfamily J, member 11 antibody, anti BIR antibody, anti HHF2 antibody, anti KIR6.2 antibody, anti MGC133230 antibody, anti PHHI antibody, anti TNDM3 antibody, anti ATP-sensitive inward rectifier potassium channel 11 antibody, anti beta-cell inward rectifier subunit antibody, anti inwardly rectifying potassium channel KIR6.2 antibody, anti potassium inwardly-rectifying channel J11 antibody

orb19676 (0.1ug/ml) staining of Human Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19676(3.8ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

KCNJ11 / KATP /

NCBI Gene ID

3767