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Catalog Number | orb19676 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to KCNJ11 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Human |
Dilution range | ELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 3-5µg/ml |
Conjugation | Unconjugated |
MW | 43.5 |
Target | KCNJ11 / KATP / |
Entrez | 3767 |
Protein Sequence | AEDPAKPRYRARQ |
RRID | AB_10755718 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti KCNJ11 antibody, anti IKATP antibody, anti po Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Pancreas shows variable staining across the islet of Langerhans. Recommended concentration, 3-5ug/ml.WB: Approx 45-48kDa band observed in Human Skeletal Muscle lysates (calculated MW of 43.5kDa according to NP_000516.3). Recommended concentration: 0.1-0.3 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb19676 (0.1ug/ml) staining of Human Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb19676(3.8ug/ml) staining of paraffin embedded Human Pancreas. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.