Goat anti-KCNJ1 / ROMK Antibody

• Catalog Number: orb20131
• Category: Antibodies
• Description: Goat polyclonal antibody to KCNJ1
• Target: KCNJ1 / ROMK
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DQININFVVDAGNEN
• RRID: AB_10755886
• MW: 44.8; 42.7
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:8000, WB: 1-3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx. 45kDa band observed in Human, Mouse and Rat Kidney lysates (calculated MW of 44.8kDa according to NP_000211.1). Recommended concentration: 1-3 μg/ml.
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti KCNJ1 antibody, anti potassium inwardly-recti
• Note: For research use only
• Entrez: 3758

orb20131 (1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.