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KCNB1/DRK1 Antibody

SKU: orb19639

Description

Goat polyclonal antibody to KCNB1

Research Area

Neuroscience

Images & Validation

Tested ApplicationsELISA
Dilution RangeELISA: 1:4000, WB: 0.5 μg/ml
Predicted ReactivityBovine, Canine, Human, Mouse, Porcine
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Preliminary experiments gave a 100kDa band and additional stronger bands at approx 50kDa and 38kDa in Human Brain (Cerebellum) and Heart lysates after 0.5 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the extra bands we observe given the calculated size of 95.9kDa according to NP_004966.1. Both detected extra bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

ClonalityPolyclonal
TargetKCNB1 / DRK1
Protein SequenceHQYIDADTDDEGQ
Molecular Weight95.9
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti KCNB1 antibody, anti potassium voltage-gated channel, Shab-related subfamily, member 1 antibody, anti DRK1 antibody, anti KV2.1 antibody, anti h-DRK1 antibody, anti delayed rectifier potassium channel Kv2.1 antibody, anti h-DRK1 K channel antibody, anti potassium channel protein DRK1 antibody, anti potassium voltage-gated channel subfamily B member 1 antibody, anti voltage-gated potassium channel antibody

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NCBI Gene Details

No NCBI Gene data available

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Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

KCNB1/DRK1 Antibody (orb19639)

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Available Sizes

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100 μg
$ 360.00
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Free Secondary Antibody (20 ul)0/0

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