Goat anti-KCC3 / SLC12A6 Antibody

• Catalog Number: orb19653
• Category: Antibodies
• Description: Goat polyclonal antibody to SLC12A6
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 2-4µg/ml
• Conjugation: Unconjugated
• MW: 128; 122; 121; 127; 126
• Target: KCC3 / SLC12A6
• Entrez: 9990
• Protein Sequence: SRHIDVCSKTKE
• RRID: AB_10748833
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti SLC12A6 antibody, anti KCC3 antibody, anti so
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Heart shows staining of myocardial fibrils in transverse section. Recommended concentration, 2-4ug/ml.WB: Approx 120kDa band observed in Human Heart and Kidney lysates (calculated MW ranging 121-128kDa according to NP_598408.1; NP_005126.1; NP_001035959.1; NP_001035960.1; NP_001035961.1; NP_001035962.1). Recommended concentration: 0.1-0.3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19653 (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19653 (2.5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.