Goat anti-KCC3 / SLC12A6 Antibody

SKU: orb19653

Description

Goat polyclonal antibody to SLC12A6

Research Area

Epigenetics, Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 2-4µg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Heart shows staining of myocardial fibrils in transverse section. Recommended concentration, 2-4ug/ml.WB: Approx 120kDa band observed in Human Heart and Kidney lysates (calculated MW ranging 121-128kDa according to NP_598408.1; NP_005126.1; NP_001035959.1; NP_001035960.1; NP_001035961.1; NP_001035962.1). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: KCC3 / SLC12A6
• Protein Sequence: SRHIDVCSKTKE
• Molecular Weight: 128; 122; 121; 127; 126
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti SLC12A6 antibody, anti KCC3 antibody, anti solute carrier family 12 (potassium/chloride transporters), member 6 antibody, anti ACCPN antibody, anti DKFZP434D2135 antibody, anti KCC3A antibody, anti KCC3B antibody, anti agenesis of corpus callosum and peripheral neuropathy (Andermann syndrome) antibody, anti potassium chloride cotransporter 3 antibody, anti potassium chloride c antibody

orb19653 (0.1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19653 (2.5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

KCC3 / SLC12A6

NCBI Gene ID

9990