Karyopherin (importin) beta 1 Antibody

SKU: orb19255

Description

Goat polyclonal antibody to KPNB1

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.03-0.1 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 90-100kDa band observed in lysates of cell lines Daudi, HEK293, and Jurkat (calculated MW of 97.2kDa according to NP_002256.2). Recommended concentration: 0.03-0.1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Karyopherin (importin) beta 1
• Molecular Weight: 97.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti karyopherin (importin) beta 1 antibody, anti HGNC:6400 antibody, anti KPNB1 antibody, anti IMB1 antibody, anti IPOB antibody, anti Impnb antibody, anti MGC2155 antibody, anti MGC2156 antibody, anti MGC2157 antibody, anti NTF97 antibody, anti importin 90 antibody, anti importin beta-1 subunit antibody, anti karyopherin beta 1 antibody, anti nuclear factor p97 antibody, anti KapB1 antibody, anti IPO1 antibody

Immunohistochemical staining of paraffin embedded Human Spleen using KPNB1 antibody.

Western blot analysis of A431 (A), HEK293 (B), Jurkat (C), Daudi (D), HeLa (E) and Kelly (F) and KNRK (G) cell lysate using KPNB1 antibody.

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells using KPNB1 antibody.

Flow cytometric analysis of paraformaldehyde fixed A431 cells using KPNB1 antibody.

Quick Database Links

Gene Symbol

Karyopherin (importin) beta 1

NCBI Gene ID

3837