KAP1/TRIM28 Antibody

• Catalog Number: orb18424
• Category: Antibodies
• Description: Goat polyclonal antibody to TRIM28
• Target: KAP1 / TRIM28
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: SSQELSGGPGDGP
• RRID: AB_10751873
• MW: 88.5
• Tested applications: ELISA, FC, IF, IHC
• Dilution range: ELISA: 1:2000, IHC-P: 2-4 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: This product has been successfully used on Human gastric cancer surgical specimens: Yokoe et al, Ann Surg Oncol. 2009 Nov 7. [Epub ahead of print]. (PMID: 19898899). Recommended concentration: 5ug/ml.WB: Approx 100-120kDa band seen in HepG2 lysate. Recommended for use at 0.2-1 μg/ml (Predicted MW approx 92kDa according to NP_005753).Experiment Notes: Immunofluorescence: Successfully used on mouse embryo at the late zygote stage, 5ug/ml (personal communication).
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti TRIM28 antibody, anti KAP1 antibody, anti TF1
• Research Area: Epigenetics, Infectious Diseases
• Note: For research use only
• Entrez: 10155

orb18424 staining (0.5ug/ml) of HepG2 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Immunohistochemical analysis of formalin-fixed and paraffin embedded Human Breast using KAP1 antibody

Immunohistochemical staining of Human Breast using TRIM28 antibody

Western blot analysis of HepG2 lysate using TRIM28 antibody