KAP1/TRIM28 Antibody

SKU: orb18424

Description

Goat polyclonal antibody to TRIM28

Research Area

Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC
• Dilution Range: ELISA: 1:2000, IHC-P: 2-4 μg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: This product has been successfully used on Human gastric cancer surgical specimens: Yokoe et al, Ann Surg Oncol. 2009 Nov 7. [Epub ahead of print]. (PMID: 19898899). Recommended concentration: 5ug/ml.WB: Approx 100-120kDa band seen in HepG2 lysate. Recommended for use at 0.2-1 μg/ml (Predicted MW approx 92kDa according to NP_005753).Experiment Notes: Immunofluorescence: Successfully used on mouse embryo at the late zygote stage, 5ug/ml (personal communication).

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: KAP1 / TRIM28
• Protein Sequence: SSQELSGGPGDGP
• Molecular Weight: 88.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti TRIM28 antibody, anti KAP1 antibody, anti TF1B antibody, anti RNF96 antibody, anti TIF1B antibody, anti tripartite motif-containing 28 antibody, anti KRAB-associated protein 1 antibody, anti nuclear corepressor KAP-1 antibody, anti transcriptional intermediary factor 1-beta antibody, anti FLJ29029 antibody, anti KRAB-associated protein 1 antibody, anti tripartite motif-containing 28 protein antibody

orb18424 staining (0.5ug/ml) of HepG2 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Immunohistochemical analysis of formalin-fixed and paraffin embedded Human Breast using KAP1 antibody

Immunohistochemical staining of Human Breast using TRIM28 antibody

Western blot analysis of HepG2 lysate using TRIM28 antibody

Quick Database Links

Gene Symbol

KAP1 / TRIM28

NCBI Gene ID

10155