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JARID2 Antibody

SKU: orb20396

Description

Goat polyclonal antibody to JARID2

Research Area

Developmental Biology, Epigenetics

Images & Validation

Tested ApplicationsELISA
Dilution RangeELISA: 1:32000, WB: 1 μg/ml
Predicted ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 29kDa band in Mouse and Rat Spinal Cord and in Mouse Fetal Brain lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 137kDa according to Mouse NP_068678.1. The 29kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

HostGoat
ClonalityPolyclonal
TargetJARID2
Protein SequenceHRSAQDLRKQVSKVN
Molecular Weight139
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti JARID2 antibody, anti jumonji, AT rich interactive domain 2 antibody, anti JMJ antibody, anti OTTHUMP00000016058 antibody, anti jumonji homolog antibody, anti jumonji, AT rich interactive domain 2 protein antibody, anti jumonji-like protein antibody

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NCBI Gene Details

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Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

JARID2 Antibody (orb20396)

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Available Sizes

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100 μg
$ 330.00
DispatchUsually dispatched within 5-10 working days
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