ITCH/AIF4 Antibody

SKU: orb19579

Description

Goat polyclonal antibody to ITCH

Research Area

Apoptotic, Cancer, Epigenetics, Immunology, Infectious Diseases, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution Range: ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 10ug/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 100kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 98.7kDa according to NP_113671.3). Recommended concentration: 0.03-0.1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ITCH / AIF4
• Protein Sequence: EIKSHDLKPNGGN
• Molecular Weight: 98.7, 102.7; 86.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ITCH antibody, anti AIF4 antibody, anti itchy homolog E3 ubiquitin protein ligase (mouse) antibody, anti AIP4 antibody, anti NAPP1 antibody, anti dJ468O1.1 antibody, anti NFE2-associated polypeptide 1 antibody, anti atrophin-1 interacting protein 4 antibody, anti dJ468O1.1 (atrophin 1 interacting protein 4, AIP4) antibody, anti itchy homolog E3 ubiquitin protein ligase antibody, anti ubiquitin protein ligase ITCH antibody

orb19579 (0.03ug/ml) staining of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunofluorescence analysis of A431 cells using ITCH antibody

Immunofluorescence analysis of U2OS cells using ITCH antibody

Flow cytometric analysis of HeLa cells using ITCH antibody

Quick Database Links

Gene Symbol

ITCH / AIF4

NCBI Gene ID

83737