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Item 1 of 4
Item 1 of 4
Goat anti-ITCH / AIF4 Antibody
Catalog Number: orb19579
| Catalog Number | orb19579 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to ITCH |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, FC, IF, WB |
| Reactivity | Bovine, Canine, Human, Mouse, Rat |
| Dilution range | ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 10ug/ml |
| Conjugation | Unconjugated |
| MW | 98.7, 102.7; 86.4 |
| Target | ITCH / AIF4 |
| Entrez | 83737 |
| Protein Sequence | EIKSHDLKPNGGN |
| RRID | AB_10755714 |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | anti ITCH antibody, anti AIF4 antibody, anti itchy Read more... |
| Note | For research use only |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 100kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 98.7kDa according to NP_113671.3). Recommended concentration: 0.03-0.1 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
orb19579 (0.03ug/ml) staining of Human Brain (Frontal Cortex) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunofluorescence analysis of A431 cells using ITCH antibody
Immunofluorescence analysis of U2OS cells using ITCH antibody
Flow cytometric analysis of HeLa cells using ITCH antibody
