IRF4 (aa393-407) Antibody

SKU: orb443081

Description

Goat polyclonal antibody to IRF4

Images & Validation

• Tested Applications: ELISA, FC, IHC, WB
• Dilution range: P-ELISA: 1: 16000, WB: 0.1-0.3μg/ml, IHC-P: 5μg/ml.
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Mouse
• Application Notes:
  WB: Approx 55kDa band observed in Human Spleen and approx 52kDa in Rat Spleen lysates (calculated MW of 51.8kDa according to Human NP_002451.2 and 51.6kDa according to Human NP_001182215.1 and Rat NP_001099578.1). Recommended concentration: 0.1-0.3ug/ml. Pri. IHC: Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml.. Notes: Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: IRF4 (aa393-407)
• Molecular Weight: 51.8; 51.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

IRF4; interferon regulatory factor 4; LSIRF; MUM1; multiple myeloma oncogene 1

Immunohistochemical staining of Human Spleen using IRF4 antibody.

Western blot analysis of Human (A) and (0.1ug/ml) Rat (B) Spleen lysate using IRF4 antibody.

Flow cytometric analysis of paraformaldehyde fixed A431 cells using IRF4 antibody.

Quick Database Links

Gene Symbol

IRF4 (aa393-407)

NCBI Gene ID

3662