IRAK4 (N Terminus) Antibody

• Catalog Number: orb19015
• Category: Antibodies
• Description: Goat polyclonal antibody to IRAK4
• Target: IRAK4 (N Terminus)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Mouse
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: NKPITPSTYVRC
• RRID: AB_10751262
• MW: 51.5
• Tested applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.5-2 μg/ml, IHC-P: 3.75 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 50kDa band observed in Human epidermoid carcinoma cell line A431 and Human cervix epitheloid carcinoma cell line HeLa lysates (calculated MW of 51.5kDa according to NP_057207.1). Recommended concentration: 1-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti IRAK4 antibody, anti REN64 antibody, anti NY-
• Research Area: Epigenetics, Immunology
• Note: For research use only
• Entrez: 51135

orb19015 (1µg/ml) staining of Hela lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb19015 (3.75µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb19015 (3.75µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.