Goat anti-Il12b / Il12p40 (mouse) Antibody

• Catalog Number: orb19735
• Category: Antibodies
• Description: Goat polyclonal antibody to IL12 p40
• Target: Il12b / Il12p40 (mouse)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: EARQQNKYENYS
• RRID: AB_10928583
• MW: 38.2
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:16000, WB: 0.3-1 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 37kDa band observed in Mouse Spleen and Rat Liver lysates (calculated MW of 38.2kDa according to Mouse NP_032378.1 and 38.4kDa according to Rat NP_072133.1). Recommended concentration: 1-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti-CLMF antibody, anti-CLMF p40 antibody, anti-C
• Note: For research use only

orb19735 (1ug/ml) staining of Rat Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot analysis of Mouse (Lane 1) and Rat (Lane 2) Skin lysate using IL12 p40 antibody