Goat anti-IL-17B (aa77-89) Antibody

• Catalog Number: orb131701
• Category: Antibodies
• Description: Goat polyclonal to IL17B
• Target: IL-17B (aa77-89)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: SELAQRKCEVNLQ
• MW: 20.4
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:128000, WB: 1-3 μg/ml
• Application notes: Approx 20kDa band observed in Human Ileum lysates (calculated MW of 20.4kDa according to NP_055258.1). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 17kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field-have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reportedWB: Approx 20kDa band observed in Human Ileum lysates (calculated MW of 20.4kDa according to NP_055258.1). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 17kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IL17B; interleukin 17B; IL-17B; IL-20; NIRF; ZCYTO
• Note: For research use only
• Entrez: 27190
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Human Ileum lysate (35ug protein in RIPA buffer) using IL17B antibody