Goat anti-IFNGR1 (aa181-193) Antibody

SKU: orb107681

Description

Goat polyclonal to IFNGR1

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 0.3-1 μg/ml
• Reactivity: Human
• Application Notes:
  WB: Approx 70kDa band observed in lysates of cell line HepG2 (calculated MW of 54.4kDa according to NP_000407.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1µg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: IFNGR1 (aa181-193)
• Molecular Weight: 54.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti IFNGR1 antibody

Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

1 µg/ml staining of K562 (A) Caco-2 (B) and HepG2 (C) cell lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

2 µg/ml staining of Human Spleen lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

4 µg/ml staining of paraffin embedded Human Lung. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Lung, with no primary antibody.

Quick Database Links

Gene Symbol

IFNGR1 (aa181-193)

NCBI Gene ID

3459