Goat anti-IER5 Antibody

• Catalog Number: orb19442
• Category: Antibodies
• Description: Goat polyclonal antibody to IER5
• Target: IER5
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QPPSGGEDDDAEE
• RRID: AB_10930213
• MW: 33.7
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:2000, WB: 1-3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Customer's experiments gave a band at approx 45kDa in lysates of transfected H1299 overexpressing IER5 but not when transfected with empty vector (calculated MW of 33.7kDa according to NP_057629.1). Recommended concentration: 1-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti IER5 antibody, anti immediate early response
• Note: For research use only
• Entrez: 51278