Goat anti-ICOS Antibody

• Catalog Number: orb20334
• Category: Antibodies
• Description: Goat polyclonal to ICOS
• Target: ICOS
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: KGGQILCDLTKTKGS
• RRID: AB_10762484
• MW: 22.6
• Tested applications: ELISA, IHC
• Dilution range: ELISA: 1:32000, WB: 0.01 μg/ml, IHC-P: 3.75 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 13kDa band in Human Peripheral Blood Lymphocyte lysates after 0.01 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 22.6kDa according to NP_036224.1. The 13kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti ICOS antibody, anti inducible T-cell co-stimu
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 29851

Immunohistochemical staining of Human Tonsil using ICOS antibody

Immunohistochemical staining of Human Thymus using ICOS antibody

Immunohistochemical staining of Human Lymph Node using ICOS antibody

Immunohistochemical staining of Human Lymph Node using ICOS antibody