Goat anti-ICOS Antibody

SKU: orb20334

Description

Goat polyclonal to ICOS

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:32000, WB: 0.01 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 13kDa band in Human Peripheral Blood Lymphocyte lysates after 0.01 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 22.6kDa according to NP_036224.1. The 13kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ICOS
• Protein Sequence: KGGQILCDLTKTKGS
• Molecular Weight: 22.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ICOS antibody, anti inducible T-cell co-stimulator antibody, anti AILIM antibody, anti CD278 antibody, anti MGC39850 antibody, anti activation-inducible lymphocyte immunomediatory molecule antibody, anti inducible costimulator antibody

Immunohistochemical staining of Human Tonsil using ICOS antibody

Immunohistochemical staining of Human Thymus using ICOS antibody

Immunohistochemical staining of Human Lymph Node using ICOS antibody

Immunohistochemical staining of Human Lymph Node using ICOS antibody

Quick Database Links

Gene Symbol

ICOS

NCBI Gene ID

29851