ICAM5 precursor Antibody

• Catalog Number: orb19347
• Category: Antibodies
• Description: Goat polyclonal antibody to ICAM5
• Target: ICAM5 precursor
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: ESPAEGEVFAIQ
• RRID: AB_10749383
• MW: 97.1
• Tested applications: ELISA, IHC
• Dilution range: ELISA: 1:128000, WB: 0.05 μg/ml, IHC-P: 2-4µg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Brain (Cerebral Cortex) shows axon/dendrite staining leaving cell bodies relatively unstained. Recommended concentration, 2-4ug/ml.WB: Preliminary experiments gave an approx 200-250kDa band in lysates of Human Brain (Frontal Cortex and Hippocampus) and Mouse Brain after 0.05 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 97.2kDa according to NP_003250.2. The 200-250kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti ICAM5 antibody, anti intercellular adhesion m
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 7087

orb19347 (2.5ug/ml) staining of paraffin embedded Human Brain (Cerebral Cortex). Steamed antigen retrieval with citrate buffer pH 6, AP-staining.