Goat Human Ig lambda light chains (free and bound), conjugated with TRITC Antibody

• Catalog Number: orb21966
• Category: Antibodies
• Description: Specificity Reference Reagent Tetramethylrhodamine isothiocyanate-conjugated IgG fraction of polyclonal goat antiserum to free and bound human Ig lambda light chain. This conjugate is intended for use in direct immunofluorescence for the detection of polyclonal immunoglobulins, purified monoclonal immunoglobulins of the lambda type as well as free light chains or Bence Jones proteins of the lambda type in serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:60.
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: TRITC
• Reactivity: Human
• Form/Appearance: Fluorochrome coupled purified goat IgG lyophilized from a solution in phosphate buffered saline (pH 7.2). No preservative added, as it may interfere with the antibody activity.
• RRID: AB_10925763
• Antibody Type: Secondary Antibody
• Source: A pool of purified Bence Jones lambda proteins isolated from human urine. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Storage: The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.