Goat anti-HTR7 / 5-HT7 Antibody

SKU: orb20243

Description

Goat polyclonal antibody to HTR7

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:16000, WB: 1-3 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 50kDa band observed in lysates of cell line KELLY (calculated MW of 49.5kDa according to NP_000863.1). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: HTR7 / 5-HT7
• Molecular Weight: 49.5; 48.0; 53.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti HTR7 antibody, anti 5-hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase-coupled) antibody, anti 5-HT7 antibody, anti 5-hydroxytryptamine receptor 7 antibody, anti OTTHUMP00000020077 antibody, anti OTTHUMP00000020078 antibody, anti OTTHUMP00000020079 antibody, anti serotonin 5-HT-7 receptor antibody

orb20243 (1ug/ml) staining of KELLY lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

HTR7 / 5-HT7

NCBI Gene ID

3363