Goat anti-HSPC150 / UBE2T Antibody

• Catalog Number: orb18482
• Category: Antibodies
• Description: Goat polyclonal antibody to UBE2T
• Target: HSPC150 / UBE2T
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QLVGIEKKFHPDV
• RRID: AB_10749310
• MW: 22.5
• Tested applications: ELISA, FC, IF, WB
• Dilution range: ELISA: 1:16000, WB: 0.1-0.3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx. 22kDa band observed in lysates of cell lines K562, HEK293 and A549 (calculated MW of 22.5kDa according to NP_054895.1). Recommended concentration: 0.1-0.3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti UBE2T antibody, anti HSPC150 antibody, anti P
• Research Area: Cancer, DNA Damage, Epigenetics
• Note: For research use only
• Entrez: 29089
• Expiration Date: 12 months from date of receipt.

Primary incubation 1 hour at room temperature. Image A: K562 cell lysate at primary Ab concentration 0.1 µg/ml, Image B: THP-1 cell lysate at primary Ab concentration 0.3 µg/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

Primary incubation 1 hour at room temperature. Images A, B, C, D: A431, HEK293, HeLa, Jurkat nuclear cell lysate at primary Ab concentration 0.3 µg/ml, Image E: HepG2 nuclear cell lysate at primary Ab concentration 0.1 µg/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing strong nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing strong nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.