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HSP60/HSPD1 (aa333-344) Antibody

SKU: orb334054

Description

Goat polyclonal antibody to HSPD1

Research Area

Neuroscience

Images & Validation

Tested ApplicationsELISA, IF, IHC, WB
Dilution RangeELISA: 1:64000, WB: 0.01-0.1 μg/ml, IHC-P: 5 μg/ml
ReactivityHuman, Porcine, Rat
Predicted ReactivityBovine, Canine
Application Notes
WB: Approx 60kDa band observed in Human and Pig Heart, Kidney and Liver and in Rat Kidney lysates (calculated MW of 61.1kDa according to Human NP_002147.2 and 60.9kDa according to Pig NP_001241645.1 and Rat NP_071565.2 ). Recommended concentration: 0.01-0.1µg. IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the mitochondria of HeLa cells and in the cytoplasm of A431 cells. Recommended concentration: 10µg/ml.

Key Properties

ClonalityPolyclonal
TargetHSP60 / HSPD1 (aa333-344)
Protein SequenceNLEDVQPHDLGK
Molecular Weight61.1
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Anti-HSPD1 antibody, Anti- heat shock 60kDa protein 1 (chaperonin) antibody, Anti- CPN60 antibody, Anti- GROEL antibody, Anti- HLD4 antibody, Anti- HSP-60 antibody, Anti- HSP60 antibody, Anti- HSP65 antibody, Anti- HuCHA60 antibody, Anti- SPG13 antibody, Anti- 60 kDa chaperonin antibody, Anti- 60 kDa heat shock protein antibody, Anti- mitochondrial antibody, Anti- P60 lymphocyte protein antibody, Anti- chaperonin 60 antibody, Anti- heat shock protein 65 antibody, Anti- mitochondrial matrix prote antibody
Quality Guarantee

Quality Guarantee

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 560.00
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Free Secondary Antibody (20 ul)0/0

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