HSP60/HSPD1 (aa333-344) Antibody

• Catalog Number: orb334054
• Category: Antibodies
• Description: Goat polyclonal antibody to HSPD1
• Target: HSP60 / HSPD1 (aa333-344)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Porcine, Rat
• Predicted Reactivity: Bovine, Canine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: NLEDVQPHDLGK
• MW: 61.1
• Tested applications: ELISA, IF, IHC, WB
• Dilution range: ELISA: 1:64000, WB: 0.01-0.1 μg/ml, IHC-P: 5 μg/ml
• Application notes: WB: Approx 60kDa band observed in Human and Pig Heart, Kidney and Liver and in Rat Kidney lysates (calculated MW of 61.1kDa according to Human NP_002147.2 and 60.9kDa according to Pig NP_001241645.1 and Rat NP_071565.2 ). Recommended concentration: 0.01-0.1µg. IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the mitochondria of HeLa cells and in the cytoplasm of A431 cells. Recommended concentration: 10µg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-HSPD1 antibody, Anti- heat shock 60kDa protei
• Note: For research use only
• Entrez: 3329
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Rat Kidney lysate using HSPD1 antibody

Western blot analysis of Human Heart (Lane 1), Kidney (Lane 2) and Liver (Lane 3) lysate and in Pig Heart (Lane 4), Kidney (lane 5) and Liver (lane 6) lysate using HSPD1 antibody

Immunofluorescence analysis of HeLa cells using HSPD1 antibody

Immunohistochemical staining of Human Kidney using HSPD1 antibody