Goat anti-HP alpha chain Antibody

• Catalog Number: orb125239
• Category: Antibodies
• Description: Goat polyclonal antibody to Haptoglobin
• Target: HP alpha chain
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QCKNYYKLRTEGD
• MW: 45.2; 38.5
• Tested applications: ELISA, FC, WB
• Dilution range: ELISA: 1:32000, WB: 0.01-0.03 μg/ml
• Application notes: WB: Approx 18kDa band observed in Human Liver lysates (calculated MW of 18kDa according to alpha chain cleaved from NP_005134.1). Recommended concentration: 0.01-0.03µg/ml.
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti Haptoglobin alpha chain antibody, anti Haptog
• Note: For research use only
• Entrez: 3240

0.01 µg/ml staining of Human Liver lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.