Goat anti-HP alpha chain Antibody

SKU: orb125239

Description

Goat polyclonal antibody to Haptoglobin

Research Area

Cardiovascular Disease

Images & Validation

• Tested Applications: ELISA, FC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.01-0.03 μg/ml
• Reactivity: Human
• Application Notes:
  WB: Approx 18kDa band observed in Human Liver lysates (calculated MW of 18kDa according to alpha chain cleaved from NP_005134.1). Recommended concentration: 0.01-0.03µg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: HP alpha chain
• Protein Sequence: QCKNYYKLRTEGD
• Molecular Weight: 45.2; 38.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Haptoglobin alpha chain antibody, anti Haptoglobin alpha(1S) beta antibody, anti Haptoglobin alpha(2FS) beta antibody, anti Haptoglobin beta chain antibody, anti Haptoglobin, alpha polypeptide antibody, anti Haptoglobin, beta polypeptide antibody, anti HP antibody, anti Hp2 alpha antibody, anti HPA1S antibody, anti HPT antibody, anti Zonulin antibody

0.01 µg/ml staining of Human Liver lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Quick Database Links

Gene Symbol

HP alpha chain

NCBI Gene ID

3240