HOXA4 Antibody

• Catalog Number: orb20582
• Category: Antibodies
• Description: Goat polyclonal antibody to HOXA4
• Target: HOXA4
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QAKGPAHGLHASH
• RRID: AB_10752708
• MW: 34.5
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 36kDa band observed in Human Colon lysates (calculated MW of 34.5kDa according to NP_002132.3). Recommended concentration: 0.1-0.3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti Dfd-like protein antibody, anti homeo box A4
• Research Area: Developmental Biology
• Note: For research use only
• Entrez: 3201

orb20582 (0.1ug/ml) staining of Human Colon lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.