Goat anti-HMGI-C / HMGA2 Antibody

• Catalog Number: orb20345
• Category: Antibodies
• Description: Goat polyclonal antibody to HMGA2
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Canine, Human
• Dilution range: ELISA: 1:8000, WB: 1-3 μg/ml
• Conjugation: Unconjugated
• MW: 11.8; 11.5
• Target: HMGI-C / HMGA2
• Entrez: 8091
• Protein Sequence: KAAQKKAEATGEK
• RRID: AB_10750226
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti HMGA2 antibody, anti high mobility group AT-h
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 18kDa band observed in Human Heart lysates (calculated MW of 11.8kDa according to NP_003474.1 and 11.5kDa according to NP_003475.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Di Cello et al, Mol Cancer Res. 2008 May;6(5):743-50.; PMID: 18505920). Recommended concentration: 1-3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb20345 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.