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Catalog Number | orb18667 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to HMG20A |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
Dilution range | ELISA: 1:32000, IHC-P: 1-2 μg/ml |
Conjugation | Unconjugated |
MW | 40.1 |
Target | HMG20A |
Entrez | 10363 |
Protein Sequence | ATVREVVNRLDR |
RRID | AB_10755572 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti HMG20A antibody, anti high-mobility group 20A Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Spleen shows staining of nucleoli.. Recommended concentration, 1-2ug/ml.WB: Preliminary experiments gave an approx 55-60kDa band in Human Testis lysate at 1 μg/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 40kDda according to NP_060670. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported |
Expiration Date | 12 months from date of receipt. |
orb18667 (2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.