Goat anti-HMBS (aa160-173) Antibody

• Catalog Number: orb318745
• Category: Antibodies
• Description: Goat polyclonal antibody to HMBS
• Target: HMBS (aa160-173)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: HLEFRSIRGNLNTR
• MW: 39.3; 37.7; 34.9; 33.3
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
• Application notes: WB: Approx 40kDa band observed in lysates of cell line K562 (calculated MW of 39.3kDa according to NP_000181.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.. IHC: Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti HMBS antibody, anti hydroxymethylbilane synth
• Note: For research use only
• Entrez: 3145

Western blot analysis of K562 lysate using HMBS antibody

Immunohistochemical staining of Human Spleen using HMBS antibody