Goat anti-HMBS (aa160-173) Antibody

SKU: orb318745

Description

Goat polyclonal antibody to HMBS

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Application Notes:
  WB: Approx 40kDa band observed in lysates of cell line K562 (calculated MW of 39.3kDa according to NP_000181.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.. IHC: Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: HMBS (aa160-173)
• Molecular Weight: 39.3; 37.7; 34.9; 33.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti HMBS antibody, anti hydroxymethylbilane synthase antibody, anti PBG-D antibody, anti PBGD antibody, anti PORC antibody, anti UPS antibody, anti porphobilinogen deaminase antibody, anti porphyria, acute antibody, anti Chester type antibody, anti pre-uroporphyrinogen synthase antibody, anti uroporphyrinogen I synthase antibody, anti uroporphyrinogen I synthetase antibody

Western blot analysis of K562 lysate using HMBS antibody

Immunohistochemical staining of Human Spleen using HMBS antibody

Quick Database Links

Gene Symbol

HMBS (aa160-173)

NCBI Gene ID

3145