HIPPI Antibody

• Catalog Number: orb20221
• Category: Antibodies
• Description: Goat polyclonal antibody to IFT57
• Target: HIPPI
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: EQPQEYDDPNATISN
• RRID: AB_10750622
• MW: 49.1
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:8000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 49kDa band observed in Human and Mouse Brain lysates (calculated MW of 49.1kDa according to Human NP_060480.1 and of 48.8kDa according to Mouse NP_082956.2). Recommended concentration: 1-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IFT57; intraflagellar transport 57 homolog (Chlamy
• Research Area: Cancer, Neuroscience
• Note: For research use only
• Entrez: 55081
• Expiration Date: 12 months from date of receipt.

orb20221 (1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Testes of IFT57 Antibody