Goat anti-HEXIM1 Antibody

SKU: orb19364

Description

Goat polyclonal antibody to HEXIM1

Research Area

Cardiovascular Disease, Epigenetics, Immunology

Images & Validation

• Tested Applications: ELISA, IF, WB
• Dilution Range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 10 μg/ml
• Reactivity: Human, Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 65kDa band observed in HeLa cell lysate and using recombinant HEXIM1 protein. Data kindly provided by David Price and Jeff Cooper, University of Iowa, USA. We find an approx. 40kDa band in Human Heart lysates (calculated MW of 40.6kDa according to NP_006451.1). Recommended concentration: 0.3-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: HEXIM1
• Molecular Weight: 40.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti HEXIM1 antibody, anti hexamethylene bis-acetamide inducible 1 antibody, anti HGNC:24953 antibody, anti CLP1 antibody, anti HIS1 antibody, anti MAQ1 antibody, anti HMBA-inducible antibody, anti hexamethylene bisacetamide-inducible protein antibody, anti hexamethylene-bis-acetamide-inducible transcript 1 antibody, anti hexamthylene bis-acetamide inducible 1 antibody, anti menage a quatre 1 antibody, anti EDG1 antibody, anti FLJ13562 antibody

orb19364 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

orb19364 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

orb19364 (0.1µg/ml) staining of Human (A) and Mouse (B) Heart lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.

Western blot analysis of HeLa cell lysate and recombinant HEXIM1 using HEXIM1 antibody

Quick Database Links

Gene Symbol

HEXIM1

NCBI Gene ID

10614