Goat anti-HES4 Antibody

• Catalog Number: orb167714
• Category: Antibodies
• Description: Goat polyclonal antibody to HES4.
• Target: HES4
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: RSAAEHRKVGSRP
• MW: 26.1; 23.5
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:128000, WB: 1-3 μg/ml
• Application notes: WB: Approx 24kDa band observed in nuclear lysates of cell line HepG2 (calculated MW of 26.1kDa according to NP_001135939.1). Recommended concentration: 1-3µg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti HES4 antibody, anti hairy and enhancer of spl
• Research Area: Neuroscience
• Note: For research use only
• Entrez: 57801

Western blot analysis of HepG2 nuclear lysate (35ug protein in RIPA buffer) using HES4 antibody