GTF2IRD1 Antibody

SKU: orb19315

Description

Goat polyclonal antibody to GTF2IRD1

Research Area

Cardiovascular Disease, Developmental Biology, Epigenetics

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution Range: ELISA: 1:64000, WB: 0.3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave bands at approx 30kDa and 40kDa in Human Heart lysate after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 106kDa according to NP_057412.1 and NP_005676.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

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Key Properties

• Clonality: Polyclonal
• Target: GTF2IRD1
• Protein Sequence: NKFTKDTTKLEPAS
• Molecular Weight: 106 105; 108
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti GTF2IRD1 antibody, anti GTF2I repeat domain containing 1 antibody, anti HGNC:4661 antibody, anti CREAM1 antibody, anti GTF3 antibody, anti MUSTRD1 antibody, anti RBAP2 antibody, anti WBSCR11 antibody, anti WBSCR12 antibody, anti hMusTRD1alpha1 antibody, anti GTF2I repeat domain-containing 1 antibody, anti Williams-Beuren syndrome chromosome region 11 antibody, anti general transcription factor 3 antibody, anti muscle TFII-I repeat antibody

Quick Database Links

Gene Symbol

GTF2IRD1

NCBI Gene ID

9569