GRAF/ARHGAP26 Antibody

SKU: orb18780

Description

Goat polyclonal antibody to ARHGAP26

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:64000, WB: 0.3-1 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 90kDa band observed in Human Heart lysates (calculated MW of 92.2kDa according to NP_055886.1). Recommended concentration: 0.3-1 μg/ml. An additional strong band of unknown identity was also consistently observed at 16kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: GRAF / ARHGAP26
• Protein Sequence: KTGLIPENYVEFL
• Molecular Weight: 92.2; 86.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ARHGAP26 antibody, anti GRAF antibody, anti Rho GTPase activating protein 26 antibody, anti KIAA0621 antibody, anti FLJ42530 antibody, anti OPHN1L antibody, anti OPHN1L1 antibody, anti GTPase regulator associated with FAK antibody, anti GTPase regulator associated with focal adhesion kinase pp125(FAK) antibody, anti GTPase regulator associated with the focal adhesion kinase pp125 antibody

orb18780 (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Colon using ARHGAP26 antibody

Quick Database Links

Gene Symbol

GRAF / ARHGAP26

NCBI Gene ID

23092