Goat anti-GRAF / ARHGAP26 Antibody

• Catalog Number: orb18780
• Category: Antibodies
• Description: Goat polyclonal antibody to ARHGAP26
• Target: GRAF / ARHGAP26
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: KTGLIPENYVEFL
• RRID: AB_10767134
• MW: 92.2; 86.2
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:64000, WB: 0.3-1 μg/ml, IHC-P: 3.75 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 90kDa band observed in Human Heart lysates (calculated MW of 92.2kDa according to NP_055886.1). Recommended concentration: 0.3-1 μg/ml. An additional strong band of unknown identity was also consistently observed at 16kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti ARHGAP26 antibody, anti GRAF antibody, anti R
• Note: For research use only
• Entrez: 23092

orb18780 (0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Colon using ARHGAP26 antibody