GPRIN3 Antibody

SKU: orb20628

Description

Goat polyclonal antibody to GPRIN3

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:4000, WB: 1-3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 80kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 82.4kDa according to NP_938022.2). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: GPRIN3
• Protein Sequence: TLEENRQTKT
• Molecular Weight: 82.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti FLJ42625 antibody, anti G protein-regulated inducer of neurite outgrowth 3 antibody, anti GPRIN family member 3 antibody, anti GRIN3 antibody, anti GPRIN3 antibody

orb20628 (1ug/ml) staining of Human Hippocampus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

GPRIN3

NCBI Gene ID

285513