GPRIN3 Antibody

• Catalog Number: orb20628
• Category: Antibodies
• Description: Goat polyclonal antibody to GPRIN3
• Target: GPRIN3
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: TLEENRQTKT
• RRID: AB_10751322
• MW: 82.4
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:4000, WB: 1-3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 80kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 82.4kDa according to NP_938022.2). Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti FLJ42625 antibody, anti G protein-regulated i
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 285513

orb20628 (1ug/ml) staining of Human Hippocampus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.